In this free webinar, attendees will gain insights into the role
of hybrid liquid chromatography-mass spectrometry (LC-MS/MS) as an
increasingly valuable tool for the bioanalysis of antibody-drug
conjugates (ADCs) to enhance the drug development process.
Attendees will also hear about the bioanalytical challenges
associated with ADCs, the different types of modalities that are
classified as ADCs and different strategies for developing "ADC
assays."
TORONTO, May 6, 2024
/PRNewswire-PRWeb/ -- Recently, the role of hybrid liquid
chromatography-mass spectrometry (LC-MS/MS) in the analysis of
antibody-drug conjugates has increased due to the complexity and
number of bioanalytical assays needed to support this modality in
the drug development process. Antibody-drug conjugates tend to
consist of a toxic small molecule payload attached via a linker to
an antibody. The application of these drugs had mainly been in
oncology where their mode of action is for the antibody to attach
at the desired target site with subsequent release of the small
molecule to combat tumor cells.
Depending on the type of conjugation and
linker used (site-specific vs Cys or Lys conjugation and cleavable
vs non-cleavable) and what reagents are available, hybrid LC-MS/MS
offers several strategies or advantages to monitor the various
constituent parts of antibody-drug conjugates.
Recently, a much wider application or use of "ADCs" to other
modalities such as antibody-peptide conjugates, antibody-RNA
conjugates (ARCs) or even other more complicated multi-functional
entities has been reported. Traditionally, LC-MS/MS has mainly been
applied to the quantitation of the small molecule payload or the
linker moiety. Ligand-binding assays are highly successful for the
quantitation of the total antibody and sometimes may be suitable
for the quantification of intact antibody-drug conjugates.
More recently, a trend of performing all analyses on LC-MS/MS
has been observed. Hybrid LC-MS/MS can easily be used to monitor
both the total antibody as well as the antibody-drug conjugates.
Depending on the type of conjugation and linker used (site-specific
vs Cys or Lys conjugation and cleavable vs non-cleavable) and what
reagents are available, hybrid LC-MS/MS offers several strategies
or advantages to monitor the various constituent parts of
antibody-drug conjugates.
This also gives scientists flexibility for the quantitation of
antibody-drug conjugates. In many cases, ligand binding or hybrid
LC-MS/MS can be applicable. However, poor reagents or limited
availability of reagents can limit the immediate application of
ligand binding. The ability to approach the analysis from different
angles to gain multiple insights into the analyte can increasingly
nudge the decision towards the use of LC-MS/MS.
In many preclinical cases, "generic" methods that make the
method development and analysis much quicker and more
cost-effective are used. At the very least, the availability of
hybrid LC-MS/MS as an alternative technology greatly increases the
chances of success in the bioanalysis of antibody-drug
conjugates.
Hybrid LC-MS/MS is proving to be an increasingly valuable tool
for delivering answers about complex analytes such as antibody-drug
conjugates to enhance the drug development process. Register for
this webinar today to get an overview of the bioanalysis of
antibody-drug conjugates.
Join featured speaker Dawn
Dufield, Scientific Officer, Mass Spectrometry, KCAS Bio,
for the live webinar on Tuesday, May 21,
2024, at 1pm EDT (10am PDT).
For more information, or to register for this event, visit
Hybrid LC-MS/MS Technology Becoming the New Norm in the Bioanalysis
of Antibody-Drug Conjugates – Examples and Case Studies.
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