SAN ANTONIO, June 6, 2016 /PRNewswire/ -- At the 64th
ASMS Conference, Bruker introduces
rapifleX™, the highest performance
MALDI-TOF/TOF mass spectrometer, which had been shown as
work-in-progress to early adopters at HUPO 2015. The
rapifleX is by far the most advanced TOF/TOF system today,
and was re-designed from the ground up to meet today's highest
demands for in-depth intact and top-down protein characterization,
and high-performance, high-throughput mass spectrometry imaging
(MSI).
With its next-generation TOF/TOF ion optics and
smartbeam® 10 kHz proprietary laser
technology, the rapifleX system now offers significantly
higher speed, better mass resolution and mass accuracy, and a
significantly enhanced MS/MS mass range to enable new research and
routine applications. Its unprecedented 10 kHz speed and ion
source robustness, its wide dynamic range, higher specificity and
resolution all contribute to the detailed characterization of
biologically and clinically relevant lipids, peptides and proteins,
resetting customer MALDI-TOF/TOF expectations for in-depth protein
characterization and imaging of tissues, cell cultures, or other
applications. Its enhanced ease-of-use, robustness and
stability make the rapifleX a game-changer for research and
large-scale validation.
Dr. Julian D. Langer, at the
Max-Planck Institute (MPI) of Biophysics and the MPI for Brain
Research in Frankfurt am Main,
Germany, stated: "In our
lab, with its high MS2 isolation efficiency, resolution
and mass accuracy at high m/z values, the rapifleX TOF/TOF has been
instrumental in identifying and characterizing new subunits of
membrane protein complexes, including prokaryotic respiratory chain
complexes and antibiotic drug targets."
The rapifleX TOF/TOF is well-suited for detailed protein
characterization in life science research and biopharmaceutical
laboratories. Many protein characterization tasks can be
improved to gain better biological insight, including
- fast identification of post-translational modifications
(PTM),
- top-down protein sequencing,
- patented T3-Sequencing to identify or confirm
suspected modifications or sequence alterations near protein N- or
C-termini,
- automated disulfide analysis of intact proteins without a
priori assumptions using Bruker's unique
DisulfideDetect™ method and software, and
- disulfide scrambling and trisulfide analysis with simplicity,
speed and dynamic range.
Tissue- and cell-type specific proteomics is a major advancement
for relevant biomarker discovery and identification, and requires
MALDI imaging studies which include the identification of detected
biomarker candidates for validation and subsequent development of
targeted assays. The rapifleX extends the MALDI
Tissuetyper® imaging capabilities
with rapid, spatially resolved MS/MS data. It supports the
patented ImageID™ workflow for biomarker
discovery in MALDI imaging cohort studies, by obtaining matching
identifications of tryptic peptides by LC-MALDI-TOF/TOF
analysis.
About the rapifleX MALDI-TOF/TOF Mass
Spectrometer
The rapifleX integrates a novel, 10 kHz smartbeam
3D scanning laser for breakthrough mass-spec imaging (MSI) with
dramatically improved spatial resolution, image contrast and
quality, and a new ion source for superior robustness and
throughput in MSI, pharma screening, as well as for anatomical
pathology clinical research.
Unique to the rapifleX is that its laser and ion optics
can be dynamically adapted to research or analytical needs.
Its smartbeam 3D laser offers different focus profiles for
highest performance in different workflows. The MS/MS ion optics
can be removed from the ion path to achieve uncompromised MS
ease-of-use for imaging, intact protein and peptide
applications. The three stage grid-less ion reflector is
software adjustable to MS/MS and intact molecular weight
measurements to achieve best performance for top-down protein
sequencing, as well as for MS/MS analysis up to 8 kDa.
Its 10-bit digitizer improves the dynamic range for quantitative
applications, such as High Throughput Screening (HTS) in drug
discovery, or quantification of scrambled disulfide bonds and
trisulfides in the 0.1-1% range relevant in biologics development
and QC.
The rapifleX™ MALDI-TOF/TOF system is for
research use only.
About the Bruker Corporation
For more than 50 years, Bruker has enabled scientists to make
breakthrough discoveries and develop new applications that improve
the quality of human life. Bruker's high-performance
scientific research instruments and high-value analytical solutions
enable scientists to explore life and materials at molecular,
cellular and microscopic levels.
In close cooperation with our customers, Bruker is enabling
innovation, productivity and customer success in life science
molecular research, in applied and pharma applications, in
microscopy, nano-analysis and industrial applications, as well as
in cell biology, preclinical imaging, clinical research,
microbiology and molecular diagnostics. For more information,
please visit: www.bruker.com
Photo - http://photos.prnewswire.com/prnh/20160603/375401
To view the original version on PR Newswire,
visit:http://www.prnewswire.com/news-releases/bruker-shows-innovative-rapiflex-maldi-toftof-at-asms-2016-300279688.html
SOURCE Bruker Corporation