WASHINGTON, March 4, 2019
/PRNewswire/ -- At the 15th Annual Conference of the US
Human Proteome Organization (www.ushupo.org), Bruker announces
further progress in high-sensitivity, high-throughput
plasma-proteomics using the timsTOF Pro mass
spectrometer connected to an Evosep One low-flow
chromatography system. Bruker also highlights advances in
PEAKS, Protein Metrics, and MaxQuant 4D
proteomics software supporting routine acquisition and information
extraction using large-scale accurate collision cross section (CCS)
values.
The timsTOF Pro with its unique PASEF (Parallel
Accumulation Serial Fragmentation) capability allows the
acquisition of ion mobility information at high throughput and with
ultra-high sensitivity, resulting in the large-scale calculation of
accurate collisional cross sections. Using novel 4D algorithms in
PEAKS Studio software resulted in a dramatic
increase of quantified proteins across a 192-sample
cohort of septic shock patients, acquired at a rate of 100
samples/day:
Dr. Roman Fischer, Nuffield
Department of Medicine, Project Leader - Discovery Proteomics, at
Oxford University, Oxford, UK, said: "The 4D feature matching
capability enabled by TIMS, boosts the number of quantified plasma
proteins in a single 11.5 min LC-MS/MS run with low sample amounts
injected. Collectively, there were 772 plasma proteins quantified
with 100ng injected using these short gradients, where 66% of the
identifications correspond to only 10% of the protein mass of
plasma. This demonstrates that the method can achieve sufficient
depth to discover biomarkers
on low abundance proteins. Achieving this depth on
the timsTOF Pro offers completely new possibilities to
analyze large sample cohorts of hundreds to thousands of samples
for biomarker discovery in blood plasma."
Dr. Gary Kruppa, Vice
President Proteomics at Bruker Daltonics, commented:
"A common practice to achieve greater
proteomic depth is sample pre-fractionation. However, this results
in long measurement times, which is often compensated
by multiplexing and chemical labeling. Although
isobaric labeling methods seem attractive in principle, they suffer
from ratio distortion that can skew or
compromise quantification of low-abundance proteins
between samples. A label-free quantitation approach combining short
LC gradients with high selectivity of 4D matching is available only
on the timsTOF Pro, and provides a compelling alternative
that can be applied to thousands of samples in high-throughput
proteomics."
Key new software developments shown at US HUPO 2019 include the
release of PEAKS On-line for the timsTOF Pro, a
server software that is parallelized with the ability to run on
clusters or multi-CPU machines. In combination with the new
denoising algorithm in timsTOF Pro acquisition software,
which reduces the data file size by a factor 5x to 10x without any
noticeable loss of information, the scalable performance of PEAKS
Online facilitates state-of-the-art high-throughput proteomics
experiments.
Protein Metrics Inc (PMI) also added peptide CCS
support to their Byonic database search algorithm, which is well
accepted by pharmaceutical and biopharma scientists for HCP and
other biologics characterization and discovery workflows.
Dr. Eric Carlson,
President and CEO of Protein Metrics Inc., stated: "We are thrilled
to include the analysis of timsTOF Pro and PASEF data in our
software for our customers. For applications such as
disulfide bond analysis, glycoanalysis, and host cell proteins, the
ability to include ion mobility and CCS data as the
4th dimension of separation, will provide even deeper
insights into protein samples to drive drug discovery and
development."
Please join Bruker's proteomics team today, March 4th, at 12:35 - 1:20 pm in the Regency Room for the
Bruker Lunch Seminar at US HUPO with the keynote talk:
"Unlocking the Power of the Fourth Dimension: MaxQuant for
TimsTOF Pro", by Dr. Juergen
Cox, Research Group Leader at Max Planck Institute of
Biochemistry, Martinsried, Germany.
About the timsTOF PRO with PASEF
The proprietary timsTOF Pro system uses
PASEF, enabled by Trapped Ion Mobility Spectrometry (TIMS), to
provide industry-leading data acquisition speed for shotgun
proteomics. The unique dual TIMS geometry of the timsTOF
Pro, combined with the time focusing of the ion packets in
the TIMS device, means that the speed advantage provided by PASEF
comes along with simultaneous improvements in sensitivity and
quantitation. All of these gains in speed, sensitivity and
quantitation maintain the advantages of Bruker's high-performance
QTOF mass spectrometers, including high mass resolution (resolving
power of 50,000 FWHM even at highest acquisition rates) in MS and
MS/MS mode, ppm accurate mass, and high isotopic fidelity (True
Isotopic Pattern, or TIPTM). The robust timsTOF
Pro with PASEF gives scientists the tools to dig deeper
into the complex cellular machinery with the potential to discover
low-level, biologically significant peptides or proteins, or
validate them in translational proteomics research.
About Bruker Corporation (Nasdaq: BRKR)
Bruker is enabling scientists to make breakthrough discoveries
and develop new applications that improve the quality of human
life. Bruker's high-performance scientific instruments and
high-value analytical and diagnostic solutions enable scientists to
explore life and materials at molecular, cellular and microscopic
levels. In close cooperation with our customers, Bruker is enabling
innovation, improved productivity and customer success in life
science molecular research, in applied and pharma applications, in
microscopy and nanoanalysis, and in industrial applications, as
well as in cell biology, preclinical imaging, clinical phenomics
and proteomics research and clinical microbiology. For more
information: www.bruker.com.
Media
Contact:
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Investor
Contact:
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Petra Scheffer,
Ph.D.
|
Miroslava
Minkova
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Director Marketing
Solutions
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Director, Investor
Relations &
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Bruker
Daltonics
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Corporate
Development
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T: +49 (421)
2205–2843
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Bruker
Corporation
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E:
petra.scheffer@bruker.com
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T: +1 (978) 663-3660,
ext. 1479
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E:
miroslava.minkova@bruker.com
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